step chromatography
英 [step ˌkrəʊməˈtɒɡrəfi]
美 [step ˌkroʊməˈtɑːɡrəfi]
台阶色谱法
双语例句
- Preparation of Defibrin for Injection with One Step Chromatography
一步层析分离制备注射用蛇毒去纤酶 - Study on the Separation of Gd, Tb and Dy by Step Eluting Chromatography
萃取色层梯度淋洗法分离钆铽镝的研究 - One step Purification of Tissue type Plasminogen Activator Derived from Expression by Milk Gland of Transgenic Goat Using Affinity Chromatography
应用一步亲和层析法纯化转基因山羊乳腺定位表达的组织型纤维蛋白溶酶原激活剂 - Superoxide Dismutase ( SOD) from garlic was purified with the metal chelate affinity chromatography ( MCAC), using chitosan as substrate. The SOD was purified to homogeneous in one step affinity chromatography. Its specific activity was 1191U/ mg and recover was 71%.
以壳聚糖为基质的金属螯合亲和层析法纯化大蒜SOD，得到比活为1191U/mg的酶蛋白，回收率为71%，纯化倍数为7.29。 - Methods: The acetylcholine M_2 muscarinic receptors were purified from porcine brain by single step affinity chromatography.
方法：利用亲和层析方法从猪脑中纯化毒蕈碱乙酰胆碱M2受体； - In blue dye affinity chromatography expecting to purify the protein of interest in one step, protein of interest was difficultly differentiated from mixed protein as much proteins bound to the chromatography media by non-specific affinity.
在蓝染料亲和层析一步分离中，由于全菌体蛋白中与亲和树脂发生非特异结合的成分较多，极难区分目的蛋白和杂蛋白。 - -103 solid adsorption stationary phase packed column, were used to validate the optimized results of linear temperature-programmed, one step temperature-programmed, linear temperature-programmed under different pressure for gas chromatography.
-103，验证网格搜索法对气相色谱的线性程序升温和一阶程序升温以及不同流量情况下线性程序升温等三种操作方式下的优化结果。 - This report offers a two step purification method for successfully purifying the DNA from soil and compost. The method is composed of the first step of polyvinylpolypyrrolidone+ Sephadex G200 chromatography and the second step of electroelution.
本研究通过利用polyvinylpolypyrrolidone+SephadexG200层析柱与电透析两步纯化的方法成功地纯化了来自土壤和堆肥样品的DNA。 - The purity of 97% can be achieved after two step purification of scFv including Ni metal chelating chromatography ( the first) and gel filtration ( the second).
经Ni离子金属螯合亲和层析、凝胶层析两步纯化，重组抗体的纯度达到97%。 - Extracellular phytase produced by Aspergillus awamori using technique of semi-solid cultivation was purified to homogeneity by employing an initial ultrafiltration step, followed by chromatography using anion exchange, gel filtration and chromatofocusing steps.
本研究以半固体发酵方式培养泡盛曲霉（Aspergillusawamori）生产植酸酶，通过超滤、阴离子交换层析，凝胶层析等步骤，纯化了植酸酶蛋白，分子量约为118kDa。 - In addition, it was suggested the crucial step determine the recovery of non-secretive recombinant proteins might be the process of sample pretreatment, not be the process of column chromatography.
另外，本文结果也提示，对于非分泌型重组蛋白来说，影响目的蛋白回收的最主要因素可能不是柱层析，而是样品前处理过程。 - The recombinant protein was conveniently purified in a single step by metal ( Ni2+) chelate affinity chromatography.
重组人TyrRS可以利用Ni2+柱亲和层析的方法快速方便地被一步纯化。 - The fusion protein could be purified in one step using metal chelate chromatography to homogeneity as judged by SDS PAGE. And the recovery rate was about 68%.
利用重组蛋白中的6个组氨酸（His）组成的标签进行亲和层析，经一步金属螯合亲和层析纯化后，重组蛋白在SDSpage上呈现出均一的单一条带，回收率达68%。 - Rate Model Simulation of Adsorption and Step Elution of Urokinase Using Affinity Chromatography
尿激酶亲和层析分离中吸附和洗脱过程的速率模型模拟 - Methods The nerve growth factor was purified from bovine seminal plasma by a two-step cation exchange chromatography.
方法利用牛精液为原料，进行两次阳离子交换层析。 - By the method, gallic acid could be obtained at a high purity of 97% just through two step chromatography.
经过一次逆流色谱分离，可以得到纯度在70%以上的没食子酸，第二次分离即可使其纯度达到97%以上。 - This paper reported an improved method for separation and purification of IgY from egg water-soluble fraction ( WSF) by one step elution anion-exchange chromatography.
报道了一步洗脱离子交换色谱法分离纯化蛋黄水溶性组分（WSF）中IgY的新方法。 - The paper introduces method and step of determination formic acid and acetic acid in exhaust gas by ion chromatography.
阐述了离子色谱法测定废气中甲酸、乙酸含量的方法和步聚。 - Objective: Our aim was to isolate and purify soluble antigen ( S Ag) from bovine retina by one step ion exchange chromatography.
目的：探讨一步离子交换层析法提纯牛视网膜可溶性抗原（SAg）。 - A kininogen was purified from chum salmon plasma with one step of papain affinity chromatography. The recovery, specific inhibitory activity and purification fold were 0.94%, 3.84 U/ mg and 30.36, respectively.
通过使用木瓜蛋白酶亲和柱，从鲑鱼血浆中纯化出了一种kiniongen，该纯化的回收率、比活力和纯化倍数分别为0.94%、3.84U/mg和30.36倍。 - Expression and purification of STEP and its mutants were preformed by affinity column and liquid chromatography.
STEP及其突变和截短的表达和纯化通过Ni-NTA亲和柱和液相色谱进行。 - Then, the influence of different experimental conditions on purification effect was analyzed by single factor and orthogonal experiments, and subsequently got the optimized process parameters of the two step column chromatography.
利用单因素实验和正交试验研究不同实验条件对纯化效果的影响，得到两步柱层析法的最佳工艺条件。